EVALUATION OF CHEMICALLY SYNTHESIZED SILVER NANOPARTICLES COATED ANTIBIOTICS AGAINST MULTI DRUG RESISTANT BACTERIA ISOLATED FROM OTITIS MEDIA PATIENTS

Abstract

Silver nanoparticles which have well-known antimicrobial properties, are used extensively in various medical and general applications. They have unique physical, chemical and biological properties as well as antibacterial activity. In the present study, silver nanoparticles were synthesized chemically and then different analytical techniques such as UV-visible spectroscopy, Scanning Electron Microscopy (SEM), and X-Ray Diffraction (XRD), were performed for the characterization of AgNPs. UV-visible spectroscopy gave absorption peak at 430 nm which was in the prescribed range and confirmed the synthesis of AgNPs. SEM micrograph demonstrated the morphology of AgNPs, while the XRD peak gave information about the phase purity, size, internal crystalline structure and nature of the synthesized AgNPs. On the Other hand, various pathogens were isolated such as S. aureus S. epidermidis Salmonella spp, Enterobacter spp, Provedencia spp, Proteus spp, Streptococcus pneumonia, Klebsiella spp, Edwardsiella terda ssp, Klebsiella oxytoca and Pseudomonas aeruginosa from pus samples. Antibacterial activity of multiple coated antibiotics with AgNPs and non-coated antibiotics including Amoxicillin + Clavulanic acid, Imipinem, Amikacin, Ciprofloxacin, Polymixin, Cefoxitin, Tazobactum + Piperacillin Nitrofurantoin, Fosfomycin,and Gentamycin were recorded against these bacterial isolates. Most enhanced silver nanoparticles activity was observed in combination with the antibiotics Ciprofloxicin (500% increase in potential) against Provedencia Rettgeri and Proteus Mirabilis while it was (100 % increase in potential) against S. aureus and S. epidermis, followed by (140% increase in potential) in combination with antibiotics Gentamycin against Proteus Mirabilis. Amoxicillin + Clavulanic acid and Fosfomycin in combination with silver nanoparticles also enhanced (100% increase in potential) against all Gram-negative bacterial species. Fractional Inhibitory Concentration (FIC) values for two bacterial isolates S. aureus, and E. coli showed synergistic effect.