IN VITRO STUDY AND CORRELATION OF AGE-SPECIFIC AMH LEVELS AS A PREDICTOR OF EUPLOIDY IN INFERTILE WOMEN

Abstract

Background: The frequency of abnormal chromosome numbers is estimated to be in the range of 10-30% for fertilized human eggs, the majority of which are monosomic or trisomic. These are closely linked to miscarriage, and account for almost a third of all miscarriages. This is more related to biological age than chronological age. The finite oocyte pool theory proposes that the depletion of the pool of oocytes in the right stage of growth contributes to the problem. Anti-Müllerian Hormone (AMH) is a clinical marker commonly used to determine ovarian reserve, and is associated with egg euploidy and pregnancy success rates.

Methods: A laboratory study was performed to assess euploidy rates of females of different ages through Preimplantation Genetic Diagnosis (PGD). AMH was quantified in serum samples via ELISA. Different statistical approaches such as Principal Component Analysis (PCA) and Partial Least Squares (PLS) were used to assess the relationship between age, AMH levels, and euploidy.
Objective: To assess the association between age, AMH, and euploidy rate in vitro.
Results: This study showed that increasing AMH levels have a positive effect on the rate of euploidy, especially in women between 25 and 35 years of age. Older women showed a reduction in euploidy with lower AMH levels. Age was negatively correlated with euploidy, while AMH was positively correlated. A higher ovarian reserve was associated with more euploid blastocysts. Indeed, statistical testing revealed a negative effect of age on ovarian outcomes, and a positive effect of AMH.

Conclusion: Age and AMH are important indicators of embryo euploidy. Age has a negative effect on ovarian reserve and euploidy, whereas AMH levels improve fertility. This shows the importance of AMH as a valuable tool in fertility testing and treatment.